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KMID : 0545120090190111456
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 11 p.1456 ~ p.1463
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Temperature-Dependency Urease Activity in Vibrio parahaemolyticus is Related to Transcriptional Activator UreR
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Park Kwon-Sam
Lee Soo-Jae
Chung Yong-Hyun
Lida Tetsuya
Honda Takehi
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Abstract
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Vibrio parahaemolyticus possessing urease-positive property is relatively rare, but such strains consistently exhibit the TDH-related hemolysin (TRH) gene. In this study, we examined the effects of incubation temperature on urease activity expression, using the TH3996 and AQ4673 strains where the enzyme activity is known to be temperaturedependent and -independent, respectively. In the TH3996 strain, β-galactosidase activity was 4.4-fold lower after 30oC cultivation than after 37oC in a ureR-lacZ fusion strain, but temperature dependency was not found in ureD- or nikA-lacZ fusion strains. However, ureR-, ureD-, and nikA-lacZ fusions of the AQ4673 strain was not influenced by incubation temperature. We compared the promoter sequences of ureR between the above two strains. Intriguingly, we detected mismatches of two nucleotides between the two strains located at positions -66 and -108 upstream of the methionine initiation codon for UreR. Additionally, urease activity was not affected by culture temperature at either 30oC or 37oC by allelic introduction of the AQ4673 ureR gene into the TH3996 ureR deletion mutant. Taken together, our study demonstrates that the transcriptional factor UreR is involved in the temperature dependency of urease activity, and two nucleotides within the ureR promoter region are of particular importance for the urease activity dependency of V. parahaemolyticus.
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KEYWORD
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Vibrio parahaemolyticus, urease, ureR, temperature dependency
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